• Microtome knives on page U1
• Diamond knives on page U3
• Tungsten carbide knives on page U7
• Tissue Choppers and Brain/Tissue Matrices for thicker sections of live tissue on page U8

EMS 5000 SERIES OSCILLATING TISSUE SLICERS

The EMS 4500 and 5000 Oscillating Tissue Slicers represent the most thoughtfully designed solution to sectioning either fixed or fresh tissue, as thin as 5 microns. No longer is there a need for embedding or freezing your sample, and preparation time is reduced dramatically. The risk of distortion and artefacts normally associated with these procedures is eliminated. The EMS 4500 and 5000 meet the highest standards for precision and accuracy as well meeting or exceeding the performance of units on the market costing twice as much.

Applications

The EMS 5000 series may be used for the sectioning of samples in the fields of electrophysiology of fresh tissue, immunocytochemistry and immunohistochemistry, neurophysiology, neuropathology, experimental biology, histology, cytology, botanical research, microscopy, polymer and materials science. The EMS 5000 now features a new design of the slicer head which minimises Z-axis vibration. This improvement dramatically improves slice surface integrity and is especially beneficial in the field of fresh tissue where IR/DIC imaging methods are concerned. The unique blade holder has now been modified to accept razor, sapphire, diamond and glass blades.

Programmable travel window

To reduce time between slices the EMS 5000 series features a user programmable travel window (cutting window). This allows the sectioning cutting range of the blade to be customised to the specimen sample. This feature of “automatic sectioning” offers a beginning advance and end advance, further automating the EMS 5000. In conjunction with the “home button” the programmable window guarantees rapid sectioning of even the largest of specimens. As well, the EMS 5000 allows the sectioning of samples in two different modes. In single slice mode, the unit sections samples and harvests them while the blade repositions itself to perform the next slice, and in the multiple slice mode the EMS 5000 will automatically perform multiple uniform slices.

Control panel

The EMS 5000 series control panel has been totally redesigned to allow for ambidextrous and ergonomic operation. The keypad has been sealed to avoid buffer spill seepage. The tray is made from moulded clear polycarbonate for improved viewing of the slicing operation. The tray is removable to facilitate specimen mounting and cleaning. Two white LEDs are installed in the blade head, which provide constant cold illumination of the specimen during the slicing process.

Slicing

The EMS 5000 series is engineered for maximum stability, which ensures even the most delicate of tissue to be cut successfully every time. The knife holder allows any blade (as per list) to be secured and the blade angle set from 10- 20 degrees. The specimen mount is x, y, z axis adjustable and the knife advance is continuously adjustable with a touch of the keypad from 0- 5mm/s and the section thickness is now adjustable in 1 micron increments ranging from 1-999 microns. The precision slicer features automatic touch selection of section thickness, blade speed, advance and a user-defined travel window, so that the consecutive sections may be cut easily and rapidly. Other EMS 5000 features include touch pad control of the blade height, slice counter / slice thickness, precise bar graph displays of blade oscillation and advance speeds. Blade oscillation is adjustable from 50-5000 cycles/minute and advance speed is adjustable continuously from 100 microns - 5mm/sec.

Mounting of specimens
The tissue pedestal with pivotal pin (included with media tray), allows for the mounting of a specimen directly to the pedestal and the ability to change the angle of your specimen relative to the blade, offering you perfect specimen orientation. The sample vice with pivotal pins (also included in package) allows for the adherence of your specimen directly to a mounting block.

Maintenance
Our EMS 5000 series is designed for easy operation and maintenance. The specimen collecting tray is removable and easy to clean. All controls may be set with one hand with options for multiple slicing modes.

Accessories
The unit comes complete with gooseneck magnifying lens, media tray (63mm wide) and pedestal, specimen vice holder, blocks, as well as a foot switch with 1.8m cable. A large tray (89mm wide) is available as an option and comes complete with fixed stage and adjustable pedestals.

EMS 4500 Tissue Slicer
The EMS 4500 is a scaled down version of the EMS 5000. It has the same specifications as the EMS 5000. The difference between the two units is that the EMS 4500 does not include the following features and accessories:
Adjustable window
Multi-slice function
Home functionality

Foot switch
Tissue blocks
Vice holder
The EMS 4500 can be upgraded to the complete EMS 5000 unit at any time.

Features and specifications

UTS-4500 EMS 4500 Oscillating tissue slicer, 110-230V ask Availability
UTS-5000 EMS 5000 Oscillating tissue slicer, 110-230V ask Availability

EMS VIBRATING MICROTOMES
The EMS 5000mz is a very competitively priced high precision unit with a z-axis deflection of only 1-2 microns and a blade advance controllable to 10 microns/sec. The EMS 7000smz unit has a sub-micron z-axis deflection and a blade advance controllable to 10 microns/second. On both units the section thickness step size is 0.001mm, and each vibrating microtome is supplied with its own z-axis calibration verifier. All types of sectioning are possible, including sectioning for visual patching of neurological tissue, heart, and lung.

Z-axis deflection
The effect of excessive Z-axis deflection on the health and viability of the tissue preparation has been much discussed since the publication of Jonas et al (2003), and the EMS 7000smz will now deliver perfect sections every time with sub-micron Z-axis deflection across a wide range of vibration speeds and amplitudes.The affordable 5000mz offers almost the same features with Z-axis deflection of 1-2 microns at all vibration speeds and amplitudes, which is less than most other microtomes on the market.

User interface
The all-new user interface is both easy to use and versatile. The 7000smz and the 5000mz offer similar operation at the press of a button. The versatility includes simple operation at the push of a button or a range of changeable and programmable parameters, and a menu enables the settings of your own preferences.

Long working life and consistency
The EMS 7000smz also gives longevity of performance, in other words it not only gives submicron performance, but the advanced vibrating mechanism does not contain bearings and other components subject to wear. Consequently it will retain the sub-micron Z-axis deflection performance for years to come, giving consistency in biological preparation.

Optional accessories
Tissue cooling is accomplished by the use of either an ice-water bath or an electronically controlled thermo-electric cooler. Other options include an LED cold light source and a magnifier or a stereoscope for clear observation whilst slicing.

Features

• Includes a Z-axis devification device
• Z-axis blade adjust minimiser
• Custom blade holder with angle set to user requirement
• Set START and STOP position for blade travel
• Vibration speeds from 50 to 120 Hz
• Amplitudes from 0.5mm to 2.25mm
• Controlled blade advance at 10 microns per sec.
• Ice water bath easily removed for cleaning
• Optional LED light source
• Optional magnifier for clear observation
• Optional stereoscope, choice of x5-x10 fixed or x10-40 zoom for optimal observation
  On the EMS 7000smz additional features:
• Choice of manual or automatic operation
• Auto programming by storage of the first slicing speed and distance profile

Specifications and optional accessories

UVM-5000 EMS 5000mz Vibrating microtome ask Availability
UVM-7000 EMS 7000smz Vibrating microtome, programmable ask Availability

SAPPHIRE KNIVES
Sapphire knives may be used with all vibrating or oscillating types of microtomes for applications in Neuroscience, Electrophysiology/Histology. They offer several advantages over conventional razor blades.

Improved section quality
Sapphire knives provide significantly improved section quality over razor blades, particularly in electrophysiology applications. Distortion-free sections are possible at section thickness down to 5µm.

Smoother surfaces for histology
By making a cleaner cut, the surface cells remain alive and intact, so the researcher can more easily explore the live and responsive interior cells. The clean cut produced by the sapphire knife results in smoother surfaces, making it easier to follow small labelled elements.

Thinner sections are possible with a vibrating microtome
The sapphire knife fits tightly in knife holders of most vibrating microtomes. Now your vibrating microtome is an attractive alternative to the cryostat when you need thin sections of fresh or lightly fixed tissue for:

• Fluorescent labelling
• Autoradiography
• Immunohistochemistry

Extend the life of your knife
Used properly, your sapphire knife edge will last for thousands of sections. The knife is easily cleaned of glue and tissue and.
Knife edges are resharpenable and are restored to original condition. Approximate time for sharpening and shipping overseas is one month.

Use older specimens
Sapphire knives easily section through myelination in mature specimens.

Specifications
Material: synthetic single crystal sapphire
Edge length: 40mm
Blade height: 12mm
Blade thickness: 0.9mm

US121 Sapphire knife, 40mm long AUD853.00 Availability
US121R Sapphire knife, 40mm long, resharpened AUD475.00

TIMIMG BELT FOR VIBRATOMES 1500 & 3000

Cat. No.	Width (mm)	Diameter (mm)	Circumference (mm)	Phase (crest to crest) (mm)
U0708-TB	3	70	220	2

Measurements are approximate.

U0708-TB Timing belt 84MXL025 for Vibratomes 1500/ 3000 AUD75.00 Available