ProSciTech: Page J1: Aurion Immunogold Reagents

IMMUNOGOLD REAGENTS & ACCESSORIES
Immunogold Products from ProSciTech

Our immunogold products are prepared using the highest quality antibodies and binding agents. All antibodies and antibody fragments have been immuno-affinity purified and immuno-cross absorbed to minimise aspecific reactions.

Performance of the reagents is measured using a dot-spot test as described by Moeremans et al (J. Immunol. Methods, 74, (1984), 353) whereas size and size distribution are checked by electron microscopy. Products are monitored at regular intervals to check for antibody release from the gold particle surface.

Immuno gold products are stored refrigerated and we ship these with ice.

AURION PRODUCT INFORMATION : EM REAGENTS

For single and multiple labelling in Electron Microscopy.

AURION reagents are supplied in 10mM phosphate buffered saline (PBS, pH 7.6), containing 1% BSA and 0.1% NaN₃.
AURION IMMUNO GOLD reagents have a guaranteed shelf life of 18 months from the date of quality control analysis. All shipments have at least six months before the expiry date.
Gold particle sizes: 6, 10, 15, 25 nm
Particle population is monodisperse resulting in the least possible size variation and overlap.
Coefficient of Variance:
- <12% for 6 and 25 nm
- <10% for 10 and 15 nm
OD_{520 nm} of 1.0 to warrant cluster free storage
Approximate gold concentration for 6nm is 100 micrograms/mL.

AURION'S GP-ULTRA SMALL REAGENTS
Smallest particle sizes available: average gold particle size below 1nm the premium reagent:
- for the highest sensitivity
- for maximum penetration
- more gold particles per secondary antibody, instead of more antibodies per gold particles
They are built around ultra small atomic gold clusters with a number of atoms/cluster <30 and an average diameter of <0.8nm.
Offers new possiblities for pre-embedding localisation of intracellular antigens.
For Light and Electron Microscopy. Used with the AURION R-Gent Silver Enhancment systems
Universal applicability: one set of reagents for any application.
Full package size accommodates the labelling of 600 slides or 1200 grids.
Reduced package sizes accommodates the labelling of 240 slides or 480 grids.
Reagents are supplied with 10 ml of Cold Water Fish Skin Gelatin(40%).
Approximate gold concentration for ultra small is 100 micrograms/mL.

AURION R-GENT SILVER ENHANCEMENT
Light insensitive silver enhancement reagents for increased detection; the black silver signal does not fade.
- easily detected in bio assays
- compatible with most counterstains used in light microscopy
Easy to use, extremely delayed auto-nucleation
Used under standard laboratory light conditions.
Available in: 2x30ml or 2x250ml and consists of a separate Developer and Enhancer.

B. AURION R-GENT SE-EM- FOR ELECTRON MICROSCOPY.
These reagents are intended for the enhancement of gold particles, especially Aurion Ultra Small particles, in electron microscopical visualisation of immunodetection and in-situ hybridisation experiments. It is our belief that the Aurion R-Gent SE-EM is a significant step forward in the improvement of sensitive detection systems based on Ultra Small Gold particles.

Features:

Light insensitive
Low viscosity
Time controlled growth of particles
Easy to use
High reproducibility
Low autonucleation
Available in 30ml size which includes a ready to use enhancer solution, initiator and activator.
Recommended on-grid enhancement time is between 15 and 30 minutes depending on desired particle size.

Spectrograph displaying the signal increase generated by silver deposition on ultra small gold particles (solid line) vs. autonucleation (hatched line) with time. Y-axis: optical density at 415 nm, X-axis: time of enhancement (0-30 min). Temperature 20ºC.

Recommended on-grid enhancement time is between 15 and 30 minutes depending on desired particle size.

AURION GOLD TRACERS

ANIONIC GOLD TRACERS for the detection of positively charged compounds. Gold particles (6, 10, 15, 25nm and ultra small) coated with acetylated albumin.
CATIONIC GOLD TRACERS for the detection of negatively charged compounds. Gold particles (6, 10, 15, 25nm and ultra small) coated with methylated albumin.
INDIFFERENT GOLD TRACERS: Gold particles (6, 10, 15, 25nm and ultra small) coated with albumin.

Full package size: 10ml at an OD520 nm of approximately 2.0.
Reduced package size: 5ml at an OD520 nm of approximately 2.0.

Prices and availability for both product ranges are shown in the "J" index page.

COL-AURION
For the sensitive staining of proteins in bio assays like immuno-blotting.
A ready-to-use reagent in units of 500ml, including one vial of 10ml Tween-20.

BACKGROUND SUPPRESSION AGENTS
Background or non-specific reactions may be caused
(i) by the proteins absorbed to the gold particle surface or
(ii) by the gold particle characteristics themselves.

A. NORMAL SERUM
Normal rabbit serum is used with the rabbit antibody gold probes, normal goat serum with the goat antibody gold probes etc. to prevent non-specific absorbtion of gold probes.

B. (I) BOVINE SERUM ALBUMIN
(II) COLD WATER FISH SKIN GELATIN
These are used as additives to the incubation buffer which diminish and frequently completely prevent background reactions characteristic to gold particle based reagents. BSA is supplied as a powder in units of 25 grams. Cold Water Fish Skin Gelatin is supplied at a concentration of 40% in units of 10 ml.

C. AURION BSA-C
This acetylated and linearised form of Bovine Serum Albumin was developed and introduced by AURION in order to deal efficiently with persisting background. By an increased net negative charge and exposure of hydrophobic domains AURION BSA-C has a two-fold effect on background. It competes with negatively charged gold particles preventing charge determined background interactions and blocks hydrophobic binding sites(e.g. many plastics used in electron microscopy, hydrophobic plastics used in disposables). AURION BSA-C is supplied as a solution of 10% in 30 ml and 100 ml units.

A special AURION newsletter describes in detail the features of AURION BSA-C. A free copy is available upon request.

D. TWEEN-20
Is a non-ionic surfactant with hydrophobic alkyl side chains. Used at or above the critical micel concentration hydrophobic background is largely prevented. It is supplied in 10 ml units. It is a registered trademark of Atlas Chem. Ind. Inc.

AURION GOLD SOLS
Aurion gold sols are supplied without preservatives. Solution contains, besides the particles, remnants of the reducing agent used during production. Those remant will be <5mM.
Average particle diameters measuring 6, 10, 15, 25nm. Unique production procedures for a narrow size distribution and optimum binding properties. Monodisperse, with a coefficient of variance <15% but in most cases <10%.

SOLS are supplied at an OD 520 nm of 1 in 100ml units with detailed instructions for use.

Prices and availability for both product ranges are shown in the "J" index page.

AURION ST-US
Ultra small atomic gold clusters stabilised with low molecular weight BSA-fragments for covalent binding to (macro)molecules by e.g. glutaraldehyde cross-linking.

The reagent is supplied in 20ml units with detailed instructions.

Mouse Monoclonal anti-FITC Gold Conjugates
For the electron microscopic visualisation of FITC labels used in light microscopic specimens. For retrieval of faded FITC in DNA/RNA probes, fatty acids, and proteins.

Prices and availability for both product ranges are shown in the "J" index page.
Search for products on price page using "Control F"

IMMUNOGOLD REAGENT KITS - EM GRADE
Complete kits with the following characteristics:

Two non-overlapping gold particle sizes
Coupled to: either Goat-anti-Rabbit and Goat-anti-Mouse IgG/IgM antibodies
Affinity purified and cross adsorbed antibodies
For use with rabbit and mouse monoclonal and polyclonal primary antibodies
Acetylated serum albumin with increased exposure of negative charges
Unparalleled low background levels

Benefits:

Covering over 90% of all applications in EM

For single and double labelling in Electron Microscopy

BENEFITS OF OUR US-KIT:

FOR EM:

For the most sensitive labelling in electron microscopy

For the penetration in ultra thin cryosections resulting in enhanced detectability and sensitivity

New possibilities for pre-embedding labelling

For the labelling of at least 330 on-grid-specimens

FOR IMMUNOBLOTTING:

Highest sensitivity

Black to brown/black signal suited for low level detection where colored precipitates fail

For the immunogold labelling of 10-20 nitrocellulose immunoblot strips

THESE PRODUCTS SHOULD BE STORED AT 4°C to 8°C.
DO NOT FREEZE.