• How that Electrophoresis works

AGAROSE
Benchmark Agarose LE (Low Electroendosmosis) is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp). It's low EEO (≤ 0.13-mr ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulphate-rich negative ions. Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colourless solution at 1g:100ml H₂O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio. Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

Features

• Multi-purpose, high purity
• Green Choice, free from organic solvents
• Enhanced resolution and clarity
• Low EEO / increase electrophoretic mobility
• RNase, DNase and Protease free

Gel strength	> 1200g/cm² (1%)
Gel temperature	36°C ± 1.5°C (1.5%)
Melt temperature	88°C ± 1.5°C (1.5%)
EEO (-mr)	0.13
Moisture content	< 7%
Sulphate	<0.2%
RNase / DNase	None Detected
Protease / Endonuclease	None Detected
Storage conditions	Room Temperature

HE21-0025 Agarose LE AUD52.00 /25g Availability
HE21-0100 Agarose LE AUD128.00 /100g Availability
HE21-0500 Agarose LE AUD373.00 /500g Availability

SYNERGEL™ - AGAROSE ADDITIVE

Improving agarose gel performance

• Superior sieving - Better sieving than agarose alone, 1 gr Synergel = 2 gr agarose
• High resolution - Synergel / Agarose gels exhibit improved spatial separation and tighter banding for DNA fragments of 50-2000bp
• Easy to use - mix, boil and pour!
• No background fluorescence - The superior optical clarity permits high quality photo-documentation
• Increased load capacity - Accommodates greater quantities of DNA reducing band tailing and smearing

HE22 Synergel™, agarose additive AUD174.00 /100g Availability

FLEXIBLE UV TRANSPARENT SHEETS for handling gels, visualizing and photo documentation

Features

• Ideally sized at 216mm x 279mm
• Thin sheet easily slides beneath a gel
• Flexible sheets eliminates air bubbles
• Protects the surface of transilluminators from cuts & scratches
• Polyolefin composition allows acid washing and autoclaving without a chemical reaction
• Optical densities for the 5mm thick sheets at 254 and 300nm emission wavelengths of the transilluminator are 0.14 and 0.04 respectively

HE23-05 Gel Handler™, 5mm thick AUD80.00 /pk/10 Availability
HE23-10 Gel Handler™, 10mm thick AUD80.00 /pk/5 Availability

FLOWMESH™ SHEET
A permeable support mesh for gels and membranes.

Features

• Protects - Place FlowMesh™ above or below a gel or filter to protect from tearing, bending or folding
• Sturdy - Supports gels during incubation with nucleic acid probes, antibody probes, equilibration buffers, stain, destain solutions, etc.
• Versatile - Use as a permeable spacer for stacking as well as for staining and destaining procedures
• Access - Both sides of a container or plastic bag are assured of constant solution access, which improves the uniformity of hybridizations and binding assays.
• Convenient - FlowMesh™ is re-usable and easily cut to size.

HE24 FlowMesh™ sheet, 216mm x 279mm AUD89.00 /pk/20 Availability

GEL DRYING FRAMES™
Gel drying frames allow researchers to quickly and easily dry gels between two sheets of cellophane without the use of expensive heat/vacuum gel dryers. The 240 x 240mm frames allow for drying of larger format gels, while the 140 x 140mm frames are ideal for mini gels and other smaller formats.

Each kit includes

• 2 open frames,
• 2 solid back plates
• 12 side clips and
• 50 sheets of pre-cut cellophane

HE25-140 Gel Drying Frames™, 140 x 140mm AUD138.00 /kit Availability
HE25-240 Gel Drying Frames™, 240 x 240mm AUD166.00 /kit Availability
HE25-C140 Gel Drying Frames™ cellophane, 140 x 140mm AUD43.00 /pk/50 Availability
HE25-C240 Gel Drying Frames™ cellophane, 240 x 240mm AUD46.00 /pk/50 Availability

RAPID COOMASSIE STAIN

Features

• Fast - Complete in 30-60 minutes
• Easy - Destaining is not necessary
• Highly Sensitive - detects as little as 30ng of protein
• Quality - Bands are sharper than those stained with conventional Coomassie Blue
• Versatile - Gels can be restained with Silver

HE26 Rapid Coomassie stain AUD100.00 /100g Availability

GELSAFE SDS-PAGE PRESERVATION SYSTEM

Features

• SDS PAGE - Specially designed for this application and will hold gels up to 102mm x 69mm x 3mm
• Save time - Eliminate the time-consuming process of vacuum drying
• Maintain resolution - Creates a moist environment that allows gels to maintain their original clarity and appearance
• Easily archive gels - Unique binder (sold separately) features a locking bar to hold gels in place and a rigid cover for added protection
• Great for digital images - When loaded, GelSafe is only 4mm thick and can easily be scanned with any standard scanner
• Minimize spills - Safely review results with colleagues and advisors without concern of liquid spillage

HE27-K GelSafe SDS-PAGE preservation system AUD35.00 /kit Availability
HE27-020 GelSafe SDS-PAGE preservation system AUD131.00 /pk/20 Availability
HE27-050 GelSafe SDS-PAGE preservation system AUD291.00 /pk/50 Availability
HE27-BB GelSafe binder, blue AUD74.00 /each Availability