Hybridisation & cell culture slides/ vessels & test plates on page H5

Lab-Tek® Chamber Slide™ System: Lab-Tek® Chamber Slide™, Lab-Tek® II Chamber Slide™ and Lab-Tek® II CC2™ Chamber Slide™ Products

The growth of various cell types on plastic, soda lime glass and borosilicate coverglass were examined. The surfaces were either unmodified, coated with poly-lysine, or stably surface modified with non-biological reagents as described by Kleinfield, et al. (1988). All substrates were assembled into Lab-Tek® and Lab-Tek®II Chamber Slide™ products.

Product Features	Lab-Tek®	Lab-Tek® II	Lab-Tek® II CC2
Configurations	1, 2, 4, 8, 16 well, flask style	1, 2, 4, 8 well	1, 2, 4, 8 well
Media chamber material	Proprietary cell culture grade polystyrene (PS)	Proprietary cell culture grade polystyrene (PS)	Proprietary cell culture grade polystyrene (PS)
Slide Materials	Soda lime glass or Permanox® plastic	Soda lime glass	Soda lime glass
Cover	Clear polystyrene, no handle, facilitates microscopic viewing	Clear polystyrene, with handle for aseptic manipulations	Clear polystyrene, with handle for aseptic manipulations
Sealant	Non-toxic medical grade silicone • Remains on slide after detachment	Biocompatible acrylic adhesive • Remains with media chamber after detachment	Biocompatible acrylic adhesive • Remains with media chamber after detachment
Reagent ‘isolator’	Created by removable silicone gasket	Created by hydrophobic border	Created by hydrophobic border
Chamber separation	No separation tool required	Requires separation tools	Requires separation tools
Growth Surface	For adherent cell culture Glass slides • 6-stage high purity water wash Permanox® plastic • Cell culture treated Polystyrene • Cell culture treated	For adherent cell culture RS treated glass slide Proprietary wash	For adherent cell culture chemically modified surface that’s optimal for fastidious cells Mimics a poly-lysine coated.
Biological testing	BHK-21, Hep-2 cells	BHK-21, Hep-2 cells	Primary chick brain neurons, Hep-2 cells
Leak tested	Yes	Yes	Yes
Slide background fluorescence	Glass: Minimal fluorescence Permanox®: Minimal fluorescence Polystyrene: Autofluorescence at <485nm	Glass: • No endogenous fluorescence	Glass: • No endogenous fluorescence
Coverslip compatibility	Yes, following gasket removal	Yes	Yes
Writing surface	“Sand-blasted” frosted end	Super Frost® white printed end	SuperFrost® blue printed end

User Notes

Growth Surface	Well Size (cm²)	Visible Area (cm²)	Working Volume (mL)
1 Chamber, Glass/Permanox	9.4	9.4	2.5-4.5
2 Chamber, Glass/Permanox	4.2	4.2	1.2-2.0
4 Chamber, Glass/Permanox	1.8	1.8	0.5-0.9
8 Chamber, Glass/Permanox	0.8	0.8	0.2-0.4
16 Chamber, Glass	0.4	0.4	0.1-0.2
Flaskette, Glass	10.0	10.0	2.5-5.0
Lab-Tek® II Chamber Slide™ Specifications
Growth Surface	Well Size (cm²)	Visible Area (cm²)	Working Volume (mL)
1 Chamber, Glass, CC2	10.0	8.6	4.0-5.0
2 Chamber, Glass, CC2	4.2	4.0	2.0-2.5
4 Chamber, Glass, CC2	2.4	1.7	1.0-1.3
8 Chamber, Glass, CC2	1.2	0.7	0.5-0.6

Lab-Tek® Chamber Slide™ System Developed for microscopy of in situ cell culture. Cells grow on a standard microscope slide. Media chamber can be removed when culturing is complete. Useful for viral and mycoplasma testing, chromosome studies toxicity tests and immunocytology. Permanox® plastic and glass do not interfere with fluorescent labels. Permanox® is a special molding resin, which meets the growing requirements of most anchorage dependent cells; also excellent chemical resistance allows for cell fixation and the staining of organic solvents for EM and other techniques. Low oxygen permeation makes Permanox® ideal for anoxic cell growth. Chamber slides combine a media-containing chambered super-structure with a standard soda lime glass slide or Permanox® plastic slide, sealing the two together with a biologically inert silicone gasket. After cells are grown, the chamber and gasket are easily removed. The slide may then be fixed, stained, cover slipped, and filed with the usual convenience. These slides, once fixed and stained, can be embedded using our Slide Duplicating Mold (Cat. 70170). The Flaskette® chamber (white cap) has a glass slide sealed with a gasket. It is useful with Lowicryl K4M embedding resin, where air inhibits polymerisation of embedding resin. Bou-Gharios, G. et al. J. Microscopy, 150, pt2, 161-163 (1988). Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.

Note: The chamber slides below are made of glass, but Permanox® is available - ask

G602 Lab-Tek® glass Chamber Slide™, 1 chamber AUD231.00 /2 trays 10% discount for 6 Availability
G604 Lab-Tek® glass Chamber Slide™, 2 chambers AUD248.00 /2 trays 10% discount for 6 Available
G606 Lab-Tek® glass Chamber Slide™, 4 chambers AUD236.00 /2 trays 10% discount for 6 Availability
G608 Lab-Tek® glass Chamber Slide™, 8 chambers AUD264.00 /2 trays 10% discount for 6 Available
G609 Lab-Tek® glass Chamber Slide™, 16 chambers AUD254.00 /2 trays 10% discount for 6 Available
G610 Lab-Tek® Flaskettes®, glass AUD258.00 /2 trays 10% discount for 6 Availability

Lab-Tek® II Chamber Slide System
Lab-Tek® II Chamber Slide™ products offer optimal surface for excellent attachment and growth of cells, easily distinguished by the blue mark. This design allows for the removal of the media chamber and adhesive from the slide, using a separation device. The blue hydrophobic border is raised slightly and isolates the individual wells, and prevents cross contamination. The slide can be coversliped.

Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.

G611 Lab-Tek® II Chamber Slide™, 1 chamber AUD232.00 /2 trays 10% discount for 6 Availability
G612 Lab-Tek® II Chamber Slide™, 2 chambers AUD251.00 /2 trays 10% discount for 6 Available
G614 Lab-Tek® II Chamber Slide™, 4 chambers AUD251.00 /2 trays 10% discount for 6 Availability
G618 Lab-Tek® II Chamber Slide™, 8 chambers AUD272.00 /2 trays Availability

Lab-Tek® II CC2™ Chamber Slide System
CC2 shares several similarities with the original Lab-Tek® II Chamber Slide, however, its pronounced difference is that the glass growth surface has been chemically modified. This modification provides an optimal surface for growing fastidious cells such as primary neurons or poorly adherent cell lines. CC2 also are non-auto fluorescent, making them ideal for fluorescent applications such as FISH (fluorescent in situ hybridization). The growth surface remains stable without refrigeration.

Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.

G631 Lab-Tek® II CC2™ Chamber Slide™, 1 chamber AUD279.00 /2 trays 10% discount for 6 Available
G632 Lab-Tek® II CC2™ Chamber Slide™, 2 chambers AUD296.00 /2 trays 10% discount for 6 Available
G634 Lab-Tek® II CC2™ Chamber Slide™, 4 chambers AUD313.00 /2 trays 10% discount for 6 Available
G638 Lab-Tek® II CC2™ Chamber Slide™, 8 chambers AUD315.00 /2 trays 10% discount for 6 Available

Lab-Tek® Chambered Coverglass System
Lab-Tek® and Lab-Tek®II Chambered Coverglass products are intended for high magnification examination of living cells using an inverted microscope. The growth surface of the Lab-Tek Chambered Coverglass products are #1 Borosilicate Coverglass from American origin. The Lab-Tek® II Chambered Coverglass products are #1.5 Borosilicate Coverglass of German origin. The thin glass growth surface allows optimal optical clarity when viewing living cells with high resolution of an inverted microscope. The media chambers are available in 1, 2, 4, and 8 well formats and cannot be removed from the coverglass surface.

The Chambered Coverglass is provided sterile and ready to use.

Product Features	Lab-Tek®	Lab-Tek® II
Configurations	1, 2, 4, 8 well	1, 2, 4, 8 well
Media chamber material	Proprietary cell culture grade polystyrene (PS)	Proprietary cell culture grade polystyrene (PS): non removable
Slide Materials
Cover	Clear polystyrene, no handle, facilitates microscopic viewing	Clear polystyrene, with handle for aseptic manipulations
Sealant	Non-toxic medical grade silicone	Non-toxic medical grade silicone
Growth Surface	For adherent cell culture. Good growth with fastidious cells, e.g., neurons	For adherent cell culture. Good growth with fastidious cells, e.g., neurons
Biological testing	BHK-21, Hep-2 cells	BHK-21, Hep-2 cells
Leak tested	Yes	Yes
Slide background fluorescence	Glass: Minimal fluorescence	Glass: Minimal fluorescence
Coverslip compatibility	Yes, following gasket removal	Yes
Writing surface	none	none

Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.

G641 Lab-Tek® Chamber Coverglass™, 1 chamber AUD267.00 /2 trays 10% discount for 6 Availability
G642 Lab-Tek® Chamber Coverglass™, 2 chambers AUD269.00 /2 trays 10% discount for 6 Available
G644 Lab-Tek® Chamber Coverglass™, 4 chambers AUD284.00 /2 trays 10% discount for 6 Available
G648 Lab-Tek® Chamber Coverglass™, 8 chambers AUD284.00 /2 trays 10% discount for 6 Available

Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.

G651 Lab-Tek® II Chamber Coverglass™, 1 chamber AUD259.00 /2 trays 10% discount for 6 Available
G652 Lab-Tek® II Chamber Coverglass™, 2 chambers AUD288.00 /2 trays 10% discount for 6 Availability
G654 Lab-Tek® II Chamber Coverglass™, 4 chambers AUD304.00 /2 trays 10% discount for 6 Availability
G658 Lab-Tek® II Chamber Coverglass™, 8 chambers AUD332.00 /2 trays 10% discount for 6 Availability

Tissue culture plates are featured on page H5 Click Here

TISSUE CULTURE INSERTS (TCI)
TCI are small cylinders with a fine, permeable membrane at the base. Cells are grown within the TCI which is immersed in a culture plate with a suitable growth medium. Cells grown on the porous membrane surface exhibit improved attachment, growth, and differentiation over cells grown on plastic surfaces. The medium contacts both the top and the bottom cell layers and, as a result, cells more closely mimic in-vivo structure and function.

Features

Transparent when wet: This provides optical clarity when viewing cells either microscopically or photographically. Eliminates background interference for better cell differentiation.

High Porosity: Ensures free diffusion of nutrients to both basolateral (bottom) and apical plasma (top) membranes of attached cells closely resembling an in-vivo environment.

Solvent Resistant: The cells can be fixed/stained without the need for transferring them with standard solutions.

Low Background Fluorescence: The membrane will not interfere with fluorescent assays.

No Matrix Coating Required: Very versatile and it eliminates the coating technique.

TCIs are available in different pore sizes and membrane material:

1. Unique Anaporel ™ Membrane:
This rigid, easy to handle, inorganic membrane offers a combination of excellent transparency when wet and maximum porosity (40-50% surface porosity). The 0.2 micron membrane insures free diffusion of macromolecules with a molecular weight >0.5KD with good transparency characteristics. Anopore membranes can be removed from the polystyrene housing using the Insert removal tool. The membrane is resistant to virtually all solvents. The TCI 10mm is used with 24-well plates; the TCI 25mm is used with 6 well plates; and the 8-well strip insert is used in 96-well plates.

2. Polycarbonate Membrane Inserts:
These polycarbonate (PC) membrane inserts are available in a variety of pore sizes and diameters: 0.4, 3.0, and 8.0 micron in both 10mm and 25mm sizes. PC membranes can be used in many cell culture applications, such as chemotaxis studies where a large pore size is required. The membranes may be removed easily from the insert and are ideal for microtome sectioning.

All TCIs are TC treated, radiation sterilised, and packed individually.

G699 TCI Anopore membrane, 10mm, pore size 0.2µm AUD122.00 /pk/12 10% discount for 4 Available

NUNCLON™∆ SURFACE MULTIDISHES FOR CELL CULTURE
Sterile polystyrene dishes with lids. Useful in all areas of cell culture including scale-up and cloning.
Features

Raised well rims to lower risk of cross contamination

Excellent optical quality

Nunclon™ ∆ certified

Number of wells: 24 round

Surface: Nunclon™∆

Bottom shape: flat

Suggested working volume per well: 1mL

Culture area per well: 1.9cm²

External dimensions: 128 x 86mm

G6824 Multi-dish plates, 24-well, round, with lid AUD47.00 /pk/5 Available

EliSpot™ COATED GLASS SLIDES
EliSpot™ slides (Enzyme-Linked Immunosorbent Spot) provide a convenient, cost effective, slide-based ELISA tool. They employ the quantitative sandwich enzyme linked immunosorbent assay (ELISA) technique to identify or select specific cell immunophenotypes.
Either a specific monoclonal or polyclonal antibody for a chosen analyte may be pre-coated onto membrane coated microscope slides. Appropriately stimulated cells are pipetted into the wells and the slide is placed into a humidified 37°C CO2 incubator for a specified period of time.
During this incubation period, the immobilised antibody in the immediate vicinity of the secreting cells, binds the secreted analyte. After washing away any cells and unbound substances, a biotinylated antibody specific for the chosen analyte is added to the wells. Following a wash to remove unbound biotynylated antibody, alkaline-phosphatase conjugated to streptavidin or fluorescent streptavidin is added. Each individual colour or fluorescent spot represents an individual analyte-secreting cell. The spots can be counted with a reader system or manually, using a stereomicroscope.
Cell phenotyping assay may be developed by the application of protein arrays on films and selective binding of cells.
Features

• Uses standard 25 x 75mm slides
• Eliminates use of leaky, membrane bottom microtiter plate assays
• Reduces sample number to cost-effective reagent quantities and sample size
• Eliminates use of vacuum or centrifuge washes
• Film coating: 0.45µm PVDF allows maximum protein binding for fluorescent & colorimetric detection
• Removable chamber for scanning & reading
• Chamber housing removes cleanly & easily

ELISpot procedure using EliSpot™ slides

G670 EliSpot™ PVDF coated glass slides, 16 wells AUD208.00 /pk/8 Availability