Chinese-made coverglass on page G8

For easy handling of coverglass see vacuum wands on page T12

Most of our coverglass is packaged 100/box. It is the finest available, very flat but flexible, made from clear, optically superior borosilicate, hydrolytic class 1. glass. Polished clean and ready to use. Refractive index is 1.524 plus/minus 0.002 Ne (Hg 'e' line 546mm).To facilitate price comparisons with the frequently quoted but obsolete ounces (28g), we have included the net weight per box of some coverglass in the descriptions; this is based on dimensions and the specific weight of 2.52.
Coverslips packed for Shandon and Medite Cover-Slippers may be ordered on indent from a German manufacturer. Advise your requirements and we will furnish our competitive quotation.
Special orders must be for at least 10x100 slips; prices would be similar to comparable sizes below, but if they are a non standard size a surcharge may apply.

Standard thickness
No.0 ......... 0.08 - 0.12 mm
No.1 ......... 0.13 - 0.17 mm
No.1.5........ 0.16 - 0.19 mm
No.2 ......... 0.19 - 0.23 mm
No.3 ......... 0.28 - 0.32 mm
No.4 ......... 0.38 - 0.42 mm
No.5 ......... 0.50 - 0.60 mm

User note - Coverglass technical information

COVERGLASS SQUARES & RECTANGLES No.1 thickness
All our coverglass for standard size slides is of the optically preferred No.1 thickness - 0.13 to 0.17mm. European manufacture.

G416 Coverglass No.1, 13 x 13mm AUD35.00 /box/200 10% discount for 5 stock?
G409 Coverglass No.1, 18 x 18mm AUD5.00 /box/100 10% discount for 10 stock?
G410 Coverglass No.1, 24 x 24mm AUD9.00 /box/100 10% discount for 10 stock?
G414 Coverglass No.1, 24 x 40mm AUD15.00 /box/100 10% discount for 10 stock?
G415 Coverglass No.1, 15 x 15mm AUD6.00 /box/100 10% discount for 10 stock?
G417 Coverglass No.1, 24 x 50mm AUD14.00 /box/100 10% discount for 10 stock?
G418 Coverglass No.1, 24 x 60mm AUD18.00 /box/100 10% discount for 10 stock?
G422 Coverglass No.1, 22 x 22mm AUD8.00 /box/100 10% discount for 10 stock?
G450 Coverglass No.1, 50 x 50mm AUD51.00 /box/100 10% discount for 10 stock?
G470 Coverglass No.1, 48 x 70mm AUD48.00 /box/100 10% discount for 10 stock?

COVERGLASS SQUARES No.0 thickness

G409-0 Coverglass No.0, 18 x 18mm AUD7.00 /box/100 10% discount for 10 stock?

COVERGLASS RECTANGLES large & very large, No 1.5 thickness
Slightly thicker No 1.5 (0.155-0.185mm) coverglass is used for extra strength.

G418-15 Coverglass No.1.5, 24 x 60mm AUD17.00 /box/100 10% discount for 10 stock?
G460-15 Coverglass No.1.5, 48 x 60mm AUD60.00 /box/100 10% discount for 10 stock?
G480-15 Coverglass No.1.5, 80 x 70mm AUD86.00 /box/100 10% discount for 10 stock?

COVERGLASS CIRCLES No.1 thickness

G404 Coverglass No.1, Ø 8mm AUD14.00 /box/100 10% discount for 10 stock?
G401 Coverglass No.1, Ø 10mm AUD24.00 /box/200 10% discount for 5 stock?
G402 Coverglass No.1, Ø 13mm AUD21.00 /box/200 10% discount for 5 stock?
G403 Coverglass No.1, Ø 50mm AUD35.00 /box/100 10% discount for 10 stock?
G405 Coverglass No.1, Ø 18mm AUD22.00 /box/200 10% discount for 5 stock?
G406 Coverglass No.1, Ø 35mm AUD26.00 /box/100 10% discount for 10 stock?
G407 Coverglass No.1, Ø 24mm AUD18.00 /box/100 10% discount for 10 stock?
G408 Coverglass No.1, Ø 22mm AUD27.00 /box/200 10% discount for 5 stock?
G430 Coverglass No.1, Ø 30mm AUD19.00 /box/100 10% discount for 10 stock?
G442 Coverglass No.1, Ø 42mm AUD35.00 /box/100 10% discount for 10 stock?

COVERGLASS CIRCLES No.0 thickness

G400-0 Coverglass No.0, Ø 5mm AUD16.00 /box/100 10% discount for 10 stock?
G402-0 Coverglass No.0, Ø 13mm AUD25.00 /box/200 10% discount for 10 stock?
G404-0 Coverglass No.0, Ø 8mm AUD21.00 /box/100 10% discount for 10 stock?

COVERGLASS CIRCLES No.1.5 thickness

G420-15 Coverglass No.1.5, Ø 15mm AUD13.00 /box/100 10% discount for 10 stock?
G405-15 Coverglass No.1.5, Ø 18mm AUD15.00 /box/100 10% discount for 10 stock?

EXTRA THICK COVERGLASS No.5 thickness

G419-5 Coverglass No.5, Ø 19mm AUD46.00 /box/100 10% discount for 10 stock?

COVERSLIPS FOR LEIGHTON TUBES
Manufactured from clear-white borosilicate glass. They offer super clarity, are perfectly flat and corrosion resistant.

G390 Coverslips No.1, 13 x 54mm for Leighton tubes L6450 AUD117.00 /box/120 stock?
G395 Coverslips No.1, 10.5 x 35mm for Leighton tubes L6452 AUD73.00 /box/215 stock?

QUARTZ COVERSLIP
0.17 to 0.21mm thick, 25.4mm diameter, 1 per package.
Square 25.4mm slips and 76mm x 25.4mm slides are available, but minimum order of 10 pieces please.

All our polished quartz slides and cover glasses are manufactured from G.E. 124 Fused Quartz. They are polished to a 80/50 scratch/dig. This equates to a 5 micron or better finish.
Note: ProSciTech can supply, on indent, a range of quartz products including Flasks, Beakers, Crucibles, Wool, Frits and Tubes.

Machined quartz - Optical properties
The optical properties of fused quartz let us choose between various types, because the degree of transparency defines material purity and the method of manufacture.
Certain indicators are the UV cutoff and the presence or absence of bands at 245nm and 2.73µm. The UV cutoff varies from 155 to 175nm for a 10mm thick test sample. The presence of transition metallic impurities will move the UV cutoff towards longer wavelengths.
The curves in the figure represent the average transmissions for a 10mm thick test sample for both commercial and optical grades. Fused quartz is very efficient for the transmission of infrared radiation. Infrared transmission extends out approximately 4 micrometers, with little absorption in the “water band” at 2.73µm.

User notes - Properties of fused quartz

G382 Quartz coverslip, Ø 25.4mm AUD25.00 /each 10% discount for 10 stock?

RINZL PLASTIC COVERSLIPS
Features

• Made from clear vinyl plastic
• Unbreakable, will not scratch or cut fingers
• Used in biological and medical labs, etc.
• Thickness 0.28mm
• Refractive index of glass
• Not autofluorescent

G52-1818 Rinzl plastic coverslips, 18 x 18mm AUD14.00 /pk/100 stock?
G52-2222 Rinzl plastic coverslips, 22 x 22mm AUD14.00 /pk/100 stock?
G52-2450 Rinzl plastic coverslips, 24 x 50mm AUD15.00 /pk/100 stock?

THERMANOX COVERSLIPS
Unbreakable coverslips, these have been treated for optimum cell attachment and growth. Useful in most tissue culture applications in place of glass coverslips. Thermanox coverslips have the same thickness as No.1.5 glass coverslips. They are made of a special solvent-resistant plastic material with very low gas permeability. Cell attachment and growth are comparable to polystyrene and glass. Thermanox may be used with amyl acetate, glutaraldehyde or propylene oxide for EM preparations. Also resistant to acetone, alcohol, xylene and other commonly used solvents. Can be easily cut with scissors. Supplied sterile.

User Notes

GL081 Thermanox coverslips, 22 x 60mm AUD96.00 /pk/100 10% discount for 5 stock?
GL082 Thermanox coverslips, 24 x 30mm AUD96.00 /pk/100 10% discount for 5 stock?
GL083 Thermanox coverslips, 13mm diam. AUD96.00 /pk/100 10% discount for 5 stock?
GL084 Thermanox coverslips, 22mm diam. AUD96.00 /pk/100 10% discount for 5 stock?
GL085 Thermanox coverslips, Ø 25mm AUD96.00 /pk/100 10% discount for 5 stock?

MUSCOVITE MICA
Muscovite mica, grade V-1 according to ASTM standard D351-97, which states:
V-1: Clear - Hard, of uniform colour, flat, free from all stains and foreign inclusions, waves, cracks, buckles and other similar defects.
Discs and rectangles ready to cleave and expose a perfect surface. Very useful for SEM or AFM as a film substrate and many other lab preparations. Refractive index 1.561.
Average thickness:
G51-09 = 0.48mm, 2g = ~23pieces
G51-12 = 0.23mm, 2g = ~34 pieces
G51-25 = 0.18mm, 4 pieces = ~4g
G51-50 = 0.18mm, 1 piece = ~2g

G51-09 Muscovite mica, grade V-1, Ø 9.5mm AUD10.00 /2g 20% discount for 10 stock?
G51-12 Muscovite mica, grade V-1, Ø 12.5mm AUD10.00 /2g 20% discount for 10 stock?
G51-25 Muscovite mica, grade V-1, 25 x 75mm AUD12.00 /pk/4 20% discount for 10 stock?
G51-50 Muscovite mica, grade V-1, 50 x 75mm AUD9.00 /ea 20% discount for 10 stock?

ACLAR® FILM
see Kingsley (3) for an explanation of these points:

• separates easily from epoxy
• transparent fluorinated-chlorinated thermoplastic without volatile components
• chemically inert for all practical purposes
• cells adhere to it readily and remain attached after fixation, dehydration and critical point drying or embedding
• accepts heavy metal sputter coating
• stable in SEM; melting point 202ºC
• ACLAR® is as transparent as glass
• fluorescence microscopy possible since ACLAR® exhibits no detectable auto fluorescence
• can be sectioned without damaging ultramicrotomy knives
• considerably simplifies the preparation of cultured cells for all types of microscopy
• sterilisable
• gives flat sections
• smooth surface makes LM observations possible
• does not degrade under UV or gamma ray radiation
• used as an O₂ barrier when flat embedding methacrylate or acrylic resins

Description and Ordering of ACLAR® Film
The 1968 paper by E.B. Masurovsky (1) and R.P. Bunge was the first to describe and clearly demonstrate the useful chemical and physical properties of ACLAR® for both tissue culture and electron microscopy. It was first used in the space program and its properties were found to be valuable for biomedical research (personal communication, Dr. E. Masurovsky). Subsequent work, especially by Mawe (2), et al and the comprehensive ACLAR study by Kingsley and Cole (3) developed ACLAR's applications.
Because of its chemical inertness, non-stick property, glass clarity, flexibility and smooth surface ACLAR solves several problems in the processing of tissue culture cells during their epoxy embedding, sectioning and study.
ACLAR may be cut with scissors or blades and does not damage microtomy knives. It is unsurpassed as a moisture barrier, it is transparent to UV and is plasticiser and stabiliser free. It is nonflammable, non-ageing and has a low dielectric constant and dissipation. It is high in dielectric strength. ACLAR can be sterilised.
Fresh material may be cut on a vibrating tissue slicer, treated with HRP and then placed on ACLAR cut into a slide shape; observed under LM and if satisfactory the process for TEM may be completed.

Physical Data

Thickness	0.198mm
Clarity	Clear
Water absorption	Nil
Dimensional change (30 min. at 93ºC)	<2%
Crystalline Melting point	202 - 204ºC
Flammability	Non-flammable

Chemical Resistance Data

Acetone	No effect
100% Ethyl alcohol	No effect
Liquid Nitrogen	Remains flexible
Osmium Tetroxide	No effect
Propylene oxide	No effect

References, ACLAR® Film
1. Masurovsky EB, Bunge RP: Cover slips for long-term nerve tissue culture. Stain Technology, 43, 3, 161 - 165 (1968)
2. Mawe GM, Bresnahan JC, Beattie, , MS: Ultrastructure of HRP-labelled neurons: a comparison of two sensitive techniques. Brain Research Bulletin, 10, 551 (1983)
3. Kingsley RE, Cole NL: Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Aclar film. J of Electron Microscopy Technique, 10, 77-85 (1988)

ACLAR® is supplied in packets of ten sheets.
An 11mm disk punch is available.

GL105 ACLAR® plastic film 203 x 317mm, thickness 0.198mm AUD89.00 /pk/10 stock?

LifterSlip™ PREMIUM PRINTED COVERGLASS
LifterSlip™ offers a simple solution to the problem of hybridization non-uniformity. An innovative raised edge design provides separation and allows for even dispersal of hybridization solution between the DNA chip and coverslip. LifterSlips™ allow for increased data quality by eliminating gradients caused by floating standard coverslips on hybridization solutions. The printed bars allow for easy injection of hybridization solution under the pre-positioned coverslips. Capillary attraction ensures that LifterSlip™ stays in place. Depending on size and design configuration LifterSlip™ can hold a range of volumes.
LifterSlip™ is No.2 thickness (0.20-0.22 mm) and the bar height is 0.04-0.06 mm.

Hybridization & cell culture slides/vessels on H5

G481 LifterSlip™ coverglass, 18 x 18mm, 7.55µL AUD150.00 /pk/160 stock?
G483 LifterSlip™ coverglass, 22 x 40mm, 20.84µL AUD150.00 /pk/59 stock?
G484 LifterSlip™ coverglass, 22 x 50mm, 26.05µL AUD150.00 /pk/47 stock?
G485 LifterSlip™ coverglass, 22 x 60mm, 31.25µL AUD150.00 /pk/39 stock?
G487 LifterSlip™ coverglass, 25 x 60mm, 35.83µL AUD150.00 /pk/35 stock?

ROUND PHOTOETCHED COVERSLIPS
These coverslips have a grid pattern etched onto #1.5 (0.17–0.25mm) thickness cover slips. On the 25mm round slips there are 200 coded areas. Unique grid pattern, numbered 1 to 200 squares, each 500µm width. The other sizes have 520 coded squares. The appearance of the etched pattern is described with item G491. The squares are visible under brightfield and have high contrast when using phase contrast microscopy.
These slips are used to facilitate mounting of micromanipulated cells or chromosome spreads.
Reference: Lin, Lin-Fang and Frank H. Riddle 1981. Photoengraving of Cover Slips and Slides to Facilitate Mounting of Micromanipulated Cells or Chromosome Spreads, Exp. Res. 134:485-488.

G490 Round photoetched coverslips, Ø 25mm AUD369.00 /pk/25 10% discount for 5 stock?

SQUARE PHOTOETCHED COVERSLIPS
The gridded square coverslips are suitable for insertion into most 6 well plates and they are adaptable to most microscope mounting plates. Made from No. 2 (0.17-0.24mm) thickness cover slips. Each square has 0.6mm sides.
The unique grid pattern is identified with two letters in each square; one identifying the horizontal, the other the vertical position. The etched borders are wide and letters fill a good part of the square; they stand out clearly and allow easy counting and visualising of specimens under brightfield illumination. The etchings are translucent, but not suitable for display images of specimens on those slips.
This system facilitates observation of actively growing cells.

G491 Square photoetched coverslips, 18 x 18mm AUD226.00 /pk/25 stock?
G492 Square photoetched coverslips, 23 x 23mm AUD233.00 /pk/25 stock?

SILICON CHIPS specimen support SEM, SIMS
These may be used for mounting very fine particulate matter. They are chemically inert and make good substrates for growing or mounting cells.
They are also useful when this smooth, inert background is required and for the determination of resolution and contrast of "in-the-lens" SEMs equipped with high brightness electron emitters. Additionally, conducting specimens, or non-conducting specimens in low vacuum or low voltage SEM, may be viewed without coating.
Si chips are opaque, have low electrical resistance, are very smooth, brittle and hard like glass. Si chips are chemically quite inert. These chips are pre-cleaned, but for tissue and cell culture, additional cleaning and sterilising may be required.
Unlike glass, Si chips are crystalline and they are cut on the >111< orientation. The supplied material is a pre-cut 100mm wafer, which is easily separated into about 187 pieces of 5x7mm.

Properties
Orientation (111)
Resistance 1-30 Ohms
Type P (Boron) if 1 primary flat Type N (Phosphor) if also a secondary flat, 45 degrees from primary flat, is present
No SiO2 top coating
Wafer thickness is 460 - 530µm
Wafer is polished on one side
After dicing they are rinsed in DI water for cleaning

Peters, K.R., Working at higher magnifications in scanning electron microscopy with secondary and backscattered electrons on metal coated biological specimens and imaging macromolecular cell membrane structures, 1985 Scanning Electron Microscopy, 1985, IV, 159.
Apkarian, R.P., High-resolution signal detection of specimen-specific secondary electrons in an analytical SEM, "Proc.. 44th Ann. Meeting of the EMSA", 1986, G.W. Bailey, Ed., San Francisco Press, 658.

G540 Silicon chip wafer AUD110.00 stock?

AFM METAL SPECIMEN DISCS
High quality magnetic stainless steel (alloy 430) discs for Atomic Force Microscopy with smooth edges and consistently flat surfaces.

G530 AFM metal specimen discs, 12mm AUD85.00 /pk/100 stock?
G532 AFM metal specimen discs, 15mm AUD92.00 /pk/100 stock?